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Codex use case

Validate bulk RNA-seq inputs

Validate bulk RNA-seq inputs before differential expression.

Difficulty Intermediate
Time horizon Long-running

Use Codex with the NGS Analysis plugin to validate sample sheets, FASTQs, and references, then return MultiQC, Salmon matrices, provenance, and a short QC interpretation before differential expression.

Best for

  • Bioinformatics teams validating bulk RNA-seq inputs before differential expression.
  • Researchers who want transcript and gene-level quantification plus QC in one thread.
  • Teams that need mapping-rate, duplication, library-type, and resource-readiness review.

Contents

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    Validate bulk RNA-seq inputs

    Validate bulk RNA-seq inputs before differential expression.

    Use Codex with the NGS Analysis plugin to validate sample sheets, FASTQs, and references, then return MultiQC, Salmon matrices, provenance, and a short QC interpretation before differential expression.

    Intermediate
    Long-running

    Use Codex with the NGS Analysis plugin to validate sample sheets, FASTQs, and references, then return MultiQC, Salmon matrices, provenance, and a short QC interpretation before differential expression.

    Intermediate
    Long-running

    Related links

    Request access to GPT-Rosalind

    Best for

    • Bioinformatics teams validating bulk RNA-seq inputs before differential expression.
    • Researchers who want transcript and gene-level quantification plus QC in one thread.
    • Teams that need mapping-rate, duplication, library-type, and resource-readiness review.

    Skills & Plugins

    • NGS Analysis
      Validate sequencing inputs, run bulk RNA-seq counts and QC, and return auditable artifacts.
    Skill Why use it
    NGS Analysis Validate sequencing inputs, run bulk RNA-seq counts and QC, and return auditable artifacts.

    Starter prompt

    Use the NGS Analysis plugin. Run bulk RNA-seq FASTQ-to-count QC on the provided sample sheet, FASTQ root, transcriptome FASTA, genome FASTA, and GTF. Return: - run_manifest.json - MultiQC plus browser-safe review links - Salmon transcript- and gene-level matrices - validation and resource-readiness artifacts - a short QC interpretation that calls out mapping rate, duplication, library-type agreement, outlier samples, and anything that would block downstream differential expression
    Use the NGS Analysis plugin. Run bulk RNA-seq FASTQ-to-count QC on the provided sample sheet, FASTQ root, transcriptome FASTA, genome FASTA, and GTF. Return: - run_manifest.json - MultiQC plus browser-safe review links - Salmon transcript- and gene-level matrices - validation and resource-readiness artifacts - a short QC interpretation that calls out mapping rate, duplication, library-type agreement, outlier samples, and anything that would block downstream differential expression

    Leverage skills

    The NGS Analysis plugin includes:

    • ngs-analysis-router
    • ngs-bulk-rnaseq-counts-qc
    • ngs-runtime-env

    When you use the plugin, Codex can use all these packaged skills.

    Step-by-step guide

    1. Point Codex to a directory with the sample sheet, FASTQs, transcriptome FASTA, genome FASTA, and GTF, or provide exact file references.
    2. Run the starter prompt so Codex can validate strandedness, reference consistency, and tool readiness before execution.
    3. Open the generated MultiQC and matrix artifacts in Codex to review mapping rate, duplication, library-type agreement, and resource readiness.
    4. Continue in the same thread to fix blockers, rerun with updated metadata, or hand the resulting gene-level matrices into downstream differential expression.

    Results

    The run returns a QC-reviewed counts bundle rather than a bare quantification output. Start with the MultiQC report to identify warnings that could affect downstream interpretation. In this example, Codex surfaces FastQC sequence-content warnings alongside the run summary so the team can decide whether the observed pattern is expected for the library preparation.

    Review FastQC sequence-content warnings alongside the bulk RNA-seq run summary.

    Next, review the Salmon statistics in the same report. Mapping rates, library-type assignments, and duplication signals provide a compact readiness check before differential expression.

    Inspect Salmon alignment and library-type statistics from the generated MultiQC report.

    The resulting gene-level count matrix is saved as a reusable artifact. Open it in Codex to confirm the expected samples and features are present, then keep it with the run provenance for downstream analysis.

    Open the generated gene-level count matrix for downstream review.

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